other dapi stain cell signaling technology (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
other dapi stain cell signaling technology
Other Dapi Stain Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/other+dapi+stain+cell+signaling+technology/DAPI/10__7554_slash_elife__63060-323-27-30
Average 98 stars, based on 2136 article reviews
Other Dapi Stain Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/other+dapi+stain+cell+signaling+technology/DAPI/10__7554_slash_elife__63060-323-27-30
Average 98 stars, based on 2136 article reviews
other dapi stain cell signaling technology - by Bioz Stars,
2026-09
98/100 stars
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Staining:Article Title: Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells Article Snippet: DOI: https://doi.org/10.7554/eLife.63060 17 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody PE anti-human CD45, mouse monoclonal Biolegend Cat# 304008 1:100 RRID:AB_314396 Antibody APC mouse IgG2a k isotype control, mouse monoclonal Biolegend Cat# 400222 1:100 Antibody PE mouse IgG1, k isotype control, mouse monoclonal Biolegend Cat# 400112 1:100 RRID:AB_2847828 Antibody Alexa-Fluor 488 Goat anti-Mouse IgG, goat polyclonal Invitrogen Cat# A11001 1:1000 RRID:AB_2534069 Recombinant protein F7L6 This paper Purified from CHO_His-F7L6-Fc, available upon request from corresponding author Recombinant protein F7L6-sc This paper Purified from CHO_His-F7L6, available upon request from corresponding author Recombinant protein Wnt3a Produced in Willert lab Willert et al., 2003 Willert, 2008 Purified from CHO_Wnt3a cells Recombinant protein RSPO1 Prof. Xi He, Harvard Medical School Purified from HEK293 _Rspo1 cells Recombinant protein FLAg FP+P-L61+3 Prof. S. Angers, Toronto University, Canada Tao et al., 2019 Recombinant protein ActivinA R and D Systems Cat# 338-AC 100 ng/mL Recombinant protein BMP4 R and D Systems Cat# 314 BP 40 ng/mL Recombinant protein SCF R and D Systems Cat# 7466-SC 40 ng/mL Recombinant protein VEGF R and D Systems Cat# 293-VE 20 ng/mL Recombinant protein FGF2 StemCell Technologies Cat#78003 10 ng/mL Commercial assay or kit SuperSignal West Dura Western Blot Substrate ThermoFisher Scientific Cat# 34075 Commercial assay or kit Pierce Coomassie (Bradford) Protein Assay Kit ThermoFisher Scientific Cat# 23200 Commercial assay or kit TRIzol Reagent ThermoFisher Scientific Cat# 15596026 Commercial assay or kit Direct-zol RNA MiniPrep Kit Zymo Research Cat# R2051 Commercial assay or kit iScript Reverse Transcription Supermix Bio-Rad Cat# 1708840 Commercial assay or kit iTaq Universal SYBR Green Supermix Bio-Rad Cat# 1725120 Chemical compound, drug Zeocin ThermoFisher Scientific R25005 1 mg/mL Chemical compound, drug Puromycin ThermoFisher Scientific A1113802 4 mg/mL Chemical compound, drug Rock inhibitor Y-27631 Tocris Cat# 1254 5 mM Chemical compound, drug GSK3 inhibitor CHIR98014 Sigma-Aldrich Cat# SML1094 250 nM Chemical compound, drug ATP Sigma-Aldrich Cat# A2383 Continued on next page Gumber, Do, et al. eLife 2020;9:e63060. .. DOI: https://doi.org/10.7554/eLife.63060 18 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug D-luciferin-Potassium Salt ThermoFisher Scientific Cat# 50227 Other |